Microbiome
Metagenomics & full-length 16S sequencing services
Choose a suitable way to study microbial communities. GSN helps compare shotgun metagenomics and full-length 16S against your research objective, sample matrix, and analysis requirements.
Choose the information your study needs
The referenced full-length 16S service focuses on bacterial-community profiling. Shotgun metagenomics reads a broader range of DNA and can support taxonomy and functional-potential analysis, depending on the method and databases selected.
The approaches are not interchangeable for every question. Explain whether the priority is bacterial composition, a broader set of microbial groups, or genes and functional pathways.
Plan sample handling and controls together
Sample matrix, biomass, extraction method, storage history, and potential host DNA inform the project plan. Consider negative controls and complete metadata at collection, particularly for low-biomass material.
For comparisons between sites or treatments, use consistent sampling and handling where possible. Record sample IDs, groups, collection times, and factors that may influence community composition.
Define the analysis you will use
Sequencing files can be accompanied by taxonomic profiles, diversity summaries, and shotgun functional analysis where included in the agreed scope. Database and method selection should reflect the study objective.
Relative abundance and sequence-based identification require interpretation within the research design. If group comparisons are needed, establish replication and comparison factors before selecting statistical analyses.
DNA requirements and shipment conditions are confirmed for the chosen method. Requirements from one matrix or extraction kit should not be assumed to apply to every environmental or biological sample.
Define your deliverables
- Sequencing files and QC summaries
- Method-specific taxonomic profiles
- Diversity analysis where included
- Shotgun functional profiles and agreed comparisons
Included tests and analyses follow the approved quotation for your project.
Common questions
A few useful answers
Does full-length 16S detect every microorganism?
No. The referenced full-length 16S service focuses on bacteria. Fungal, viral, or other targets should be discussed as separate study objectives.
Does shotgun sequencing automatically include functional analysis?
Functional analysis is a bioinformatics deliverable that must be selected. Confirm its scope, databases, and result formats in the quotation.
Can soil or unprocessed source material be submitted?
Share the material type and storage history first. Acceptance, extraction, input quantity, and shipment instructions require project-specific confirmation.