Genomics
Whole genome sequencing services
Build a sequencing plan around your genomic question. GSN helps coordinate whole genome sequencing projects, from DNA requirements to the data and analysis your study needs.
Choose between short reads and HiFi long reads
Start with the purpose of the study: identifying variants against a reference, comparing isolates, or building a genome de novo. Short-read and HiFi long-read options should be compared against the biological question, DNA quality, and available budget.
CeGaT genome sequencing services include Illumina and PacBio technologies. GSN coordinates service selection and project details; the instruments and processing facilities depend on the provider agreed for the project.
Define the output before comparing quotations
Sample count, estimated genome size, ploidy, and intended analysis inform sequencing output. Gigabases describe data volume; achieved genome coverage also depends on usable reads and the properties of the sample.
Population and group-comparison projects benefit from consistent designs and complete metadata. Assembly projects require an early discussion of reference availability, expected assembly goals, and sequencing method.
Specify DNA requirements and analysis separately
DNA input requirements depend on the selected product. HiFi workflows require particular attention to long-fragment integrity. Share concentration results, a fragment profile, and the extraction method when available.
Sequencing files, mapping, variant analysis, assembly, and annotation are distinct deliverables. Identify the required outputs in the quotation so that the results match the study rather than assuming every analysis is included.
Define your deliverables
- Sequencing files and product-specific QC summaries
- Reference mapping when selected
- Variant analysis or genome assembly as options
- Annotation and reporting within the agreed scope
Included tests and analyses follow the approved quotation for your project.
Common questions
A few useful answers
How many gigabases are needed per sample?
There is no universal amount. Genome size, ploidy, study objective, sequencing method, and the agreed coverage target determine the proposed output.
Does WGS automatically include assembly?
Assembly must be specified as part of the analysis scope. Tell us whether you need raw data, mapping, variants, assembly, annotation, or a combination.
Can I submit DNA that has already been extracted?
Existing DNA can be reviewed using concentration, volume, extraction history, and integrity information. Final suitability follows QC and the selected product requirements.